In determining the function of a gene, it provides much information to observe the changes in a biological system after disruption of the gene of interest through its knockout. Thanks to the microarray technology, it is now possible to profile transcriptional changes of the whole genome, thus differentiating genes that are significantly affected by the knockout. Based on microarray experiments of hundreds of different knockouts, we assigned the so called, Perturbation Sensitivity, to the Saccharomyces cerevisiae genome by the frequency of significant changes in the transcript level in hundreds of knockout conditions. Biologically, it reflects the degree of a genes sensitivity to external perturbations.