Sumoylation is a post-translational modification (PTM) process, in which small ubiquitin-related modifier (SUMO) is attaching by covalent bonds to substrate protein. It is critical to many different biological processes such as replicating genome, expressing gene, localizing and stabilizing proteins, unfortunately, it is also involved with many major disorders including Alzheimers and Parkinsons diseases. Therefore, for both basic research and drug development, it is important to identify the sumoylation sites in proteins.