PANDA (Passing Attributes between Networks for Data Assimilation) is a gene regulatory network inference method that uses message-passing to integrate multiple sources of omics data. PANDA was originally coded in Cxe2x80x82++. In this application note we describe PyPanda, the Python version of PANDA. PyPanda runs considerably faster than the Cxe2x80x82++xe2x80x82version and includes additional features for network analysis.