#	Organism	UniProt	GHF	Temp	pH	Km	Source
1	Bacillus subtilis	I3QIG4	1	60	6	ND*	Chamoli et al. [1]
2	Uncultured bacterium (Metagenome China South Sea)	D5KX75	1	40	6.5	20.4	Yang et al. [2]
3	Uncultured bacterium (Metagenome Turpan Depression)	A0A0F7KKB7	1	50	5	ND*	Cao et al. [3]
4	Exiguobacterium antarcticum B7	K0A8J9	1	30	7	6.18	Crespim et al. [4]
5	Uncultured bacterium (Metagenome Kusaya gravy)	ND*	1	45	5-6.5	0.36	Uchiyama et al. [5]
6	Thermoanaerobacterium aotearoense	A0A0H4NXH8	1	60	6	ND*	Yang et al. [6]
7	Talaromyces funiculosus (Penicillium funiculosum)	K4KB38	3	60	5	1.25	Ramani et al. [7]
8	Humicola grisea var. thermoidea	O93784	1	40	6	ND*	de Giuseppe et al. [8]; Benoliel et al. [9]
9	Uncultured bacterium (Metagenome soil)	K4I4U1	1	50	6	2.09	Lu et al. [10]
10	Thermoanaerobacterium thermosaccharolyticum	D9TR57	1	70	6.4	7.9	Pei et al. [11]
11	Fervidobacterium islandicum	G8YZD7	1	90	7	ND*	Jabbour et al. [12]
12	Mucor circinelloides	ND*	3	50	5	4.55	Huang et al. [13]
13	Hypocrea jecorina (Trichoderma reesei)	O93785	1	30-40	5-7	2.48	Guo et al. [14]
14	Thermotoga naphthophila	D2C6W2	1	80	7	7.76	Akram et al. [15]
15	Caldicellulosiruptor bescii	B9MNR1	1	85	6.8	90.8	Bai et al. [16]
16	Neurospora crassa	U9W8B8	1	40-45	5.5-6.5	0.21	Meleiro et al. [18]
17	Pyrococcus furiosus	E7FHY4	1	> 80	ND*	ND*	Cota et al. [19]
18	Thermotoga petrophila	A5IL97	1	> 80	ND*	ND*	Cota et al. [19]
19	Acidilobus saccharovorans	D9PZ08	1	93	6	ND*	Gumerov et al. [20]
20	Uncultured bacterium (Metagenome hydrothermal spring)	W8W3B8	1	90	6.5	ND*	Schröder et al. [21]
21	Neotermes koshunensis	Q8T0W7	1	50	5	ND*	Uchima et al. [22]; de Giuseppe et al. [8]
22	Thermoanaerobacter brockii	Q60026	1	ND*	ND*	ND*	Breves et al. [23]
23	Nasutitermes takasagoensis	D0VYR9	1	65	5.5	ND*	Uchima et al. [24]

* ND not determined.

1. Chamoli S, Kumar P, Navani NK, Verma AK. Secretory expression, characterization and docking study of glucose-tolerant β-glucosidase from B. subtilis. Int. J. Biol. Macromol. 2016;85:425–33. 
2. Yang Y, Zhang X, Yin Q, Fang W, Fang Z, Wang X, et al. A mechanism of glucose tolerance and stimulation of GH1 β-glucosidases. Sci. Rep. 2015;5:17296. 
3. Cao L, Wang Z, Ren G, Kong W, Li L, Xie W, et al. Engineering a novel glucose-tolerant β-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentration. Biotechnol. Biofuels [Internet]. 2015 [cited 2016 Feb 17];8. Available from: http://www.biotechnologyforbiofuels.com/content/8/1/202
4. Crespim E, Zanphorlin LM, de Souza FHM, Diogo JA, Gazolla AC, Machado CB, et al. A novel cold-adapted and glucose-tolerant GH1 β-glucosidase from Exiguobacterium antarcticum B7. Int. J. Biol. Macromol. 2016;82:375–80. 
5. Uchiyama T, Yaoi K, Miyazaki K. Glucose-tolerant β-glucosidase retrieved from a Kusaya gravy metagenome. Front. Microbiol. [Internet]. 2015 [cited 2016 Feb 17];6. Available from: http://journal.frontiersin.org/Article/10.3389/fmicb.2015.00548/abstract
6. Yang F, Yang X, Li Z, Du C, Wang J, Li S. Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose. Appl. Microbiol. Biotechnol. 2015;99:8903–15. 
7. Ramani G, Meera B, Vanitha C, Rajendhran J, Gunasekaran P. Molecular cloning and expression of thermostable glucose-tolerant β-glucosidase of Penicillium funiculosum NCL1 in Pichia pastoris and its characterization. J. Ind. Microbiol. Biotechnol. 2015;42:553–65. 
8. de Giuseppe PO, Souza T de ACB, Souza FHM, Zanphorlin LM, Machado CB, Ward RJ, et al. Structural basis for glucose tolerance in GH1 β-glucosidases. Acta Crystallogr. D Biol. Crystallogr. 2014;70:1631–9. 
9. Benoliel B, Poças-Fonseca MJ, Torres FAG, de Moraes LMP. Expression of a Glucose-tolerant β-glucosidase from Humicola grisea var. thermoidea in Saccharomyces cerevisiae. Appl. Biochem. Biotechnol. 2010;160:2036–44. 
10. Lu J, Du L, Wei Y, Hu Y, Huang R. Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome. Acta Biochim. Biophys. Sin. 2013;45:664–73. 
11. Pei J, Pang Q, Zhao L, Fan S, Shi H. Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose. Biotechnol Biofuels. 2012;5:1–10. 
12. Jabbour D, Klippel B, Antranikian G. A novel thermostable and glucose-tolerant β-glucosidase from Fervidobacterium islandicum. Appl. Microbiol. Biotechnol. 2012;93:1947–56. 
13. Huang Y, Busk PK, Grell MN, Zhao H, Lange L. Identification of a β-glucosidase from the Mucor circinelloides genome by peptide pattern recognition. Enzyme Microb. Technol. 2014;67:47–52. 
14. Guo B, Amano Y, Nozaki K. Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis. Silman I, editor. PLOS ONE. 2016;11:e0147301. 
15. Akram F, Haq I ul, Khan MA, Hussain Z, Mukhtar H, Iqbal K. Cloning with kinetic and thermodynamic insight of a novel hyperthermostable β-glucosidase from Thermotoga naphthophila RKU-10T with excellent glucose tolerance. J. Mol. Catal. B Enzym. 2016;124:92–104. 
16. Bai A, Zhao X, Jin Y, Yang G, Feng Y. A novel thermophilic β-glucosidase from Caldicellulosiruptor bescii: Characterization and its synergistic catalysis with other cellulases. J. Mol. Catal. B Enzym. 2013;85–86:248–56. 
17. Meleiro LP, Salgado JCS, Maldonado RF, Alponti JS, Zimbardi ALRL, Jorge JA, et al. A Neurospora crassa ß-glucosidase with potential for lignocellulose hydrolysis shows strong glucose tolerance and stimulation by glucose and xylose. J. Mol. Catal. B Enzym. 2015;122:131–40. 
18. Cota J, Corrêa TLR, Damásio ARL, Diogo JA, Hoffmam ZB, Garcia W, et al. Comparative analysis of three hyperthermophilic GH1 and GH3 family members with industrial potential. New Biotechnol. 2015;32:13–20. 
19. Gumerov VM, Rakitin AL, Mardanov AV, Ravin NV, Gumerov VM, Rakitin AL, et al. A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans, A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans. Archaea Archaea. 2015;2015, 2015:e978632. 
20. Schröder C, Elleuche S, Blank S, Antranikian G. Characterization of a heat-active archaeal β-glucosidase from a hydrothermal spring metagenome. Enzyme Microb. Technol. 2014;57:48–54. 
21. Uchima CA, Tokuda G, Watanabe H, Kitamoto K, Arioka M. Heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae. Appl. Microbiol. Biotechnol. 2011;89:1761–71. 
22. Breves R, Bronnenmeier K, Wild N, Lottspeich F, Staudenbauer WL, Hofemeister J. Genes encoding two different beta-glucosidases of Thermoanaerobacter brockii are clustered in a common operon. Appl. Environ. Microbiol. 1997;63:3902–10. 
23. Uchima CA, Tokuda G, Watanabe H, Kitamoto K, Arioka M. Heterologous Expression in Pichia pastoris and Characterization of an Endogenous Thermostable and High-Glucose-Tolerant β-Glucosidase from the Termite Nasutitermes takasagoensis. Appl. Environ. Microbiol. 2012;78:4288–93.

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TCBB.2014.2325018.

S.C. Lovell, I.W. Davis, W.B. Arendall III, P.I.W. de Bakker, J.M. Word, M.G. Prisant, J.S. Richardson and D.C. Richardson (2002) Structure validation by Calpha geometry: phi,psi and Cbeta deviation. Proteins: Structure, Function & Genetics. 50: 437-450.

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